Microbiology

A bench scientist’s practical guide to isolating transfection-grade plasmid DNA using the GeneJET Miniprep Kit, alkaline lysis, EndA+ washes, and troubleshooting.

A bench scientist’s guide to extracting soluble recombinant proteins from E. coli cell pellets using 25 mM Tris, 500 mM NaCl, 0.1% Tween-20, and lysozyme.